GFP+ NPC Implant GFP and Differentiation Marker Expression. Implants were sectioned and immunostained with an antibody to GFP (a, red) to assess GFP downregulation (GFP = green, DAPI = blue).

<p>In both NPC only and high ratio NPC∶BEC groups, at all time points central clusters of GFP+ cells were surrounded by a thin border of cells that did not exhibit green fluorescence but which became visible upon staining (a). Sections were also immunostained with sox 2(b, red) to assess differentiation status of the GFP+ NPCs. Sox2 staining remained in the nuclei of GFP+ cells only over time and across treatment groups (b). Finally, sections were immunostained with GFAP to determine their differentiation status (c, red). GFAP staining overlapped with GFP+ staining and also stained a thin surrounding border of GFP- cells just beyond the central GFP+ cluster (c). Scale bars: 50 µm.