R26NotchIC Cells Lose <i>Sox1</i> and Acquire BLBP Expression

<div><p>R26NotchIC ES cells or parental control 46C cells cultured under monolayer differentiation conditions.</p> <p>(A) Proportion of <i>Sox1</i>GFP+ cells at various time points measured by FACS analysis. </p> <p>(B–G) Cultures at various time points shown in phase contrast or stained for markers as indicated.</p> <p>(H) Quantitative RT-PCR for BLBP normalised to GAPDH and displayed relative to expression in day 7 46C ES cells.</p> <p>(I) R26NotchIC cells at day 7 of monolayer differentiation stained with an antibody to GFP in order to amplify detection of <i>Sox1</i>GFP (green) together with an antibody to BLBP (red). </p> <p>(J) Schematic diagram to illustrate the transition of ES cells into <i>Sox1</i>GFP+ neuroepithelial progenitors and then into BLBP+ bipolar neural progenitors. </p>