Histological identification of microinjection sites in the ARCN.

<p>A: Microinjection site in the ARCN marked with green retrobeads IX (20 nl) (a typical site). B–D: Composite diagrams of ARCN sections at levels 3.12 mm, 3.60 mm and 3.84 mm caudal to the bregma showing microinjection sites (n = 11). Each dark spot represents one microinjection site in one animal. Calibration bar in panel A = 500 µm. Abbreviations: DMN, the hypothalamic dorsomedial nucleus; ME, the hypothalamic median eminence; PH, the posterior hypothalamic nucleus; VMN, the hypothalamic ventromedial nucleus; 3V, the third ventricle.