CTB selectively labels cell in distinct brain regions.

<p>(A) A portion of the atlas page (Lateral 0.24) from Paxinos and Franklin (2nd edition, © 2001) corresponding to the sagittal section shown in (B) and (C). The dotted line in (A) depicts where the images in (B) and (C) are cropped. CTB was injected into the VTA and a low-power image containing the arcuate nucleus was taken to reveal the distribution of CTB (B). Fluorescence from the injection site can be seen at the upper right edge of the photo in (B, marked with asterisk). (C) The same field of view as in (B) imaged to reveal the POMC-Ds-Red labeled cells in the arcuate and some of their prominent fibers rostral and caudal to the arcuate nucleus. Abbreviations: ac, anterior commissure; PaAP, paraventricular hypothalamic nucleus-anterior parvocellular; PaLM, paraventricular hypothalamic nucleus-lateral magnocellular; PaMM, paraventricular hypothalamic nucleus-medial magnocellular; PaDC, paraventricular hypothalamic nucleus-dorsal cap; PaPo, paraventricular hypothalamic nucleus-posterior; PH, posterior hypothalamic area; MPA, medial preoptic area; AHA, anterior hypothalamic area; LA, lateroanterior hypothalamic nucleus; VMH, ventromedial hypothalamic nucleus; DM, dorsomedial hypothalamic nucleus; Arc, arcuate hypothalamic nucleus, MM, medial mammilary nucleus; IPF, interpeduncular fossa; OX, optic chiasm; VTA, ventral tegmental area.