Effects of injection volume, transport time, and placement of injection.

<p>(A) Scatter plot indicating the percent of POMC-DsRed neurons containing CTB after 200 nl or 400 nl injections of CTB into the VTA. Each point represents the counts from an individual animal. (B) Plot indicating the percent of POMC-DsRed cells that contain CTB at various time-points after CTB was injected into the VTA. Each point represents the counts from an individual animal. (C) Images show POMC-DsRed cells (red) and CTB-positive cells (green) in the AHiAL (left) and the arcuate nucleus (right) after CTB was injected into the ventral striatum. There are no POMC-DsRed cells in the AHiAL and no CTB-positive cells in the arcuate nucleus. The diagram shown for reference was adapted from Paxinos and Franklin (2nd edition, © 2001). Abbreviations: BSTIA, bed nucleus of the stria terminalis- intraamygdaloid subdivision; AHiAL, amygdalohippocampal area- anterolateral; PMco, posteromedial cortical amygdaloid nucleus; DG, dentate gyrus; STh, subthalamic nucleus; LH, lateral hypothalamus; f, fornix; Arc, arcuate nucleus (MP, medial posterior; LP, lateroposterior); DM, dorsomedial hypothalamic nucleus; 3V, third ventricle; ME, median eminence.