Replication and shedding of vaccine viruses Ban/AF, B1, and LaSota in 1-day old chicks.

<p>Chicks in groups of 3 per virus were infected with virus by a single nasal drop each, as described in Materials and Methods. At 4, 6, and 8 dpi, three birds per virus per day were sacrificed, and tissue from the indicated organs, as well as oral and cloaca swabs, were collected. These were assayed for the presence of virus by inoculation into 9-day embryonated chicken eggs, as summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052751#pone-0052751-t002" target="_blank">Table 2</a>. Samples that were positive in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052751#pone-0052751-t005" target="_blank">Table 5</a> were analyzed to determine virus titers by TCID50 assay. Samples: lung (A), trachea (B), spleen (C), pancreas (D), oral swabs (E) and cloaca swabs (F).