Replication of APMVs in 2-week-old chickens.

a<p>Groups of 2-week-old chickens were inoculated with each virus by the intranasal route. Three birds from each group were sacrificed on day 4, and tissues samples (brain, trachea, lung, and spleen) were collected and homogenized. To confirm the virus replication, each sample (100 µl) in the collected samples was inoculated into three eggs, and allantoic fluids were collected on 3 dpi. Virus replication was determined by hemagglutination assay. Note that APMV-5 was not analyzed because it does not replicate in the allantoic cavity of chicken eggs.