Bim protein and mRNA levels are regulated by ABT-737.

<p>HeLa cells were treated with 10 µM ABT737 for the indicated periods. Cell extracts were analyzed by Western Blot using Bim and GAPDH antibodies (A). The expression level of Bim mRNA was detected by Real-time RT-PCR (B). HeLa cells were treated with various concentrations of ABT737 for 24 h. Cell extracts were analyzed by Western Blot using Bim and GAPDH antibodies (C). The expression level of Bim mRNA was detected by Real-time RT-PCR (D). Error bars represent standard deviations (SD) calculated from three parallel experiments (**, p<0.01 VS Control Group).