Binding of lectins to Hpt.

<p>The wells of a microtiter plate were coated with different amounts of pHpt-N (white bars), pHpt-P (grey bars), or sHpt-P (black bars). Solutions containing 1 µM biotinylated LTA (panel A), MAA (panel B), ConA (panel C), or SNA (panel D) were separately incubated into the wells. Avidin-HRP and hydrogen peroxide were used to develop color from OPD. Color intensity was determined by measuring the absorbance at 492 nm (A₄₉₂). Five equal aliqouts of each sample were processed, and means ± SEM are shown. Asterisk: significant difference between the linked bars (P<0.0001). Triangle: not significant difference (P>0.05). Data from one experiment are shown. Inter-assay CV for each sample, from three separate experiments, was less than 5%.