JSRV Env activates the MAPK pathway.

<p>(A) Representative western blot analysis of 80 µg protein extracts from the lungs of A_JEJJenv mice at 20 weeks post-infection using a panel of phospho-specific (p) antibodies. (B) Representative immunohistochemical staining of lung sections from A_JEJJenv infected mice at 20 weeks post-infection at 10x magnification unless otherwise stated. Note that the dark blue staining in some of the images represents alkaline phosphatase. (C) The ratio of protein over β-actin signal intensity was determined for each band on western blot using densitometric analysis and the average fold activation over mock infected was graphed. Values above the dotted line represent activation above mock infected.