AAV mediated expression of JSRV Env induces lung adenocarcinomas in immunocompetent mice.

<p>Illustration of A_JEJJenv, the AAV vector used to express JSRV Env in mouse lungs (A). TR, AAV terminal repeat; JE, JSRV enhancer element; Env, JSRV envelope; and PolyA, polyadenylation signal. Immunostaining for Env, SPC, and CC10 expression in lung tissue from A_JEJJenv infected NOD/SCID (B, D, F, H) and C57BL/6 (C, E, G, I) mice at 10x magnification. Representative images of hematoxylin and eosin stained lung tumors from NOD/SCID (B) and C57BL/6 (C) mice. Representative images show robust Env expression within all cells of the lung tumor (D, E). Representative images show lung tumors staining uniformly positive for SPC, a marker for alveolar type II cells (F, G), and negative for CC10, a marker for Clara cells (H, I; unstained tumors are labeled).