A loss of pyrin causes increased IL-1β protein levels in response to NLRP3 inflammasome stimuli.

<p>rpMΦs from <i>Mefv</i>^+/+ and <i>Mefv</i>^−/− littermate mice were exposed to LPS and the indicated stimuli as described in the method section. The concentration of IL-1β, <i>A</i>, and IL-6, <i>B</i>, cytokines in cell culture supernatants was determined by ELISA. <i>C</i>, LDH protein levels detected in cell supernatants. The total protein of cell lysates was measured to verify equivalent plating of cells. TiO2, titanium dioxide; MDP, muramyl dipeptide; Alum, aluminum hydroxide; CPPD, calcium pyrophosphate dihydrate; ATP, adenosine 5′ triphosphate. n = 6 mice per genotype. A student’s t test was used to calculate p values for WT versus <i>Mefv</i>^−/− cultures. #, p<0.05; *, p≤0.01 **, p≤0.001, ***, p<0.0001.