Coronal sections of opossum brain at P70 (A–C).

<p>In (A) the coronal section was Nissl-stained. In the adult opossum (B) a strong signal was predominantly present in the hippocampus. Marginal signal was also detected in the caudate nucleus (C), in the internal capsule (iC) and in the putamen (Pu). A very residual signal was observed in the external capsule (eC). In (C) the specific distribution of PrP^C in the different hippocampal layers was analyzed. Staining in the <i>lacunosum</i> and <i>moleculare</i> (lac/mol) was lower than in the <i>oriens</i> (Or) and the <i>radiatum</i> (Rad). PrP^C immunoreactivity was also observed in the <i>subiculum</i> (Sb) the main output of the hippocampus. (Bars: 0.5 mm).