Confirmation of antibody specificity and developmental expression of PrP^C in opossum CNS.

<p>(A) Western blotting of Op, MoPrP^+/+ and MoPrP^−/− whole brain homogenates confirmed the specificity of the PrP^C signal. The blot was then reprobed with β-tubulin antibody to demonstrate equal loading of samples (50 µg per lane). (B) Western blotting analysis of equal amounts of total brain homogenate (50 µg per lane) at different developmental stages showed a major electrophoretic band at 37 kDa, which corresponds to the diglycosylated form of the protein. β-tubulin was used as loading control. (C) Western blotting of indicated brain regions at different developmental stages showed a relevant change in PrP^C expression during postnatal development. Each data point represents the mean absorbance ±SEM of 3 females from different litters. All the absorbance values were normalized against β-actin.