Direct stimulation of VEGF promoter activity by Prx1 depends on Sp1 but not NF-κB or STAT-3.

<p>2H-11 cells were transfected with the VEGF promoter luciferase reporter construct (VEGF Pro), a control in which the VEGF promoter was reversed (VEGF Rev) or with VEGF promoter constructs mutated at either the (<b>A</b>) SP1 sites (S1: −75 to −61; S2: −65 to −51; S3 −52 to −38) or (<b>B</b>) NF-κB sites (N1/N2: −227 to −213; N3/4: −120 to −106). Cells were either untreated (Control) or stimulated with 20 nM Prx1 24 hours after transfection and promoter activity was measured 6 hours later. Results are presented as the average relative VEGF promoter activity; error bars represent SEM. <b>C.</b> 2H-11 cells were transfected with a control in which the VEGF promoter was reversed (VEGF Rev) or with the VEGF promoter luciferase reporter construct (VEGF Pro). Transfected cells were treated with S31–201 for 3 hours followed by treatment with 20 nM rPrx1 for 6 hours. Promoter activity was measured. Results are presented as the average relative VEGF promoter activity; error bars represent SEM. * Represents P≤0.05 when compared to untreated control levels. ** Represents P≤0.01 when compared to untreated control levels; # represents P≤0.05 when compared to VEGF Pro stimulated values.