Prx1 stimulation of endothelial cell proliferation and motility depends on NF-κB, and HIF-1.

<p><b>A.</b> HUVEC were stimulated with 20 nM Prx1 and proliferation was measured 24 hours later by SRB assay. Results are presented as the average fold proliferation over untreated control cells; error bars represent SEM. n≥3. ** Represents P≤0.001 when compared to control untreated levels. <b>B.</b> 2H-11 cells were transfected with control vectors or vectors encoding for IκB-αSR. Cells were stimulated with 20 nM Prx1 24 hours after transfection and proliferation was measured 24 hours later by MTT assay. In some cases cells were incubated with echinomycin 16 h prior to addition of Prx1. Results are presented as the average fold proliferation over untreated control cells; error bars represent SEM. n≥3. *** Represents P≤0.0001 when compared to control untreated levels; ### represents P≤0.0001 when compared to control stimulated values. <b>C.</b> 2H-11 cells were transfected with control vectors or vectors encoding for IκB-αSR or shHIF-1α. Cell migration was tested by mechanically removing cells from a section of the culture dish 24 hours after transfection and culturing the remaining cells in the absence (Unstim.) or presence of 20 nM rPrx1 for 24 hours. Images from 3 independent experiments are shown. Quantization of the number of migrated cells is shown to the right. Results are presented as the average number of cells; error bars represent SEM. n≥3. ** Represents P≤0.01 when compared to control untreated levels; ## represents P≤0.01 when compared to control stimulated values; # represents P≤0.05 when compared to control stimulated values.