HDAC6 associated with the endosomal compartments in mIMCD3 cells.

<p>(A) mIMCD3 cells were serum-starved overnight and then treated with 80 ng/ml EGF-Alexa Fluor 488 for 10 min. After that, cells treated with 0.005% saponin were stained with anti EEA1 antibody. Image in <i>zoom</i> shows the example of early endosomes stained with EEA1 (<i>red</i>) colocalized with EGFR (<i>green</i>). (B) Serum-starved mIMCD3 cells were treated with 100 ng/ml EGF for 10 min and then stained with anti-HDAC6 and EEA1 antibodies. Image in <i>zoom</i> shows the example of early endosomes stained with EEA1 (<i>red</i>) colocalized with HDAC6 (<i>green</i>). (C) mIMCD3 cells were treated and processed as in (A) except using anti-HDAC6 antibody. Image in <i>zoom</i> shows the colocalization of EGFR (<i>green</i>) with HDAC6 (red). (D) mIMCD cells were treated with 80 ng/ml EGF-Alexa Fluor 488 for 60 min and then stained with anti-LAMP2 antibody. Image in <i>zoom</i> shows the colocalization of EGFR (<i>green</i>) with later endosomes stained with LAMP2 (<i>red</i>). (E) mIMCD3 cells were treated with 100 ng/ml EGF for 60 min and then stained with anti-HDAC6 and anti-LAMP2 antibodies. Image in <i>zoom</i> shows the example of later endosomes stained with LAMP2 (<i>red</i>) colocalized with HDAC6 (<i>green</i>). (F) mIMCD3 cells were treated and processed as in (D) except using anti-HDAC6 antibody. Image in <i>zoom</i> shows the colocalization of EGFR (<i>green</i>) with HDAC6 (red).