dsRBEC selectively introduces polyIC into EGFR over-expressing cells.

<p><b>A)</b> Expression of EGFR in MDA-MB-468 and MCF7 cells was evaluated by FACS (left) or by western blot (right) as described in the Materials and Methods. <b>B)</b> Confocal live imaging of Cy3-polyIC internalization in MDA-MB-468 and MCF7 cells. Cy3-polyIC was delivered by dsRBEC (Upper row), or added directly to the cell culture medium (Lower row). The figure shows Cy3-polyIC localization at time 0 (before treatment) and after 120 minutes of treatment (Scale bar 20μm). <b>C)</b> Cy3-polyIC/dsRBEC and AlexaFluor647-transferrin were added to MDA-MB-468 cells simultaneously. Endosomal localization of Cy3-polyIC/dsRBEC is indicated by its strong co-localization with the recycling endosomal marker transferrin, 60 minutes after the start of treatment. Cy3-polyIC (red), transferrin (green), merge (yellow). (Scale bar 10μm).