The effect of lentiviral 14-3-3ε transduction on apoptosis in hippocampal neurons.

<p>Hippocampal neurons transduced with lentiviral 14-3-3ε for 48 h were treated with Hcy for 24 h. A, 14-3-3ε mRNA and proteins were analyzed by real-time quantitative PCR or western blotting, respectively. B, The percentages of neurons with apoptotic nuclei (Hoechst staining) were quantified. C, Cleaved PARP was determined by western blotting. D, Total Bad and phospho-Bad levels were assessed by western blotting and densitometric analysis. *<i>P</i><0.05 vs. control. ^#<i>P</i><0.05 vs. Hcy treatment group.