Phosphorylation of 4E-BP1 in brains of PD subjects.

<p>Western blot analysis of (<b>A</b>) frontal cortex or (<b>B</b>) basal ganglia soluble fractions from human control, idiopathic PD (iPD) and G2019S LRRK2 PD subjects with antibodies to total or phosphorylated (Thr37/46) 4E-BP1, or β-actin as a protein loading control. Molecular mass markers are indicated in kilodaltons (kDa). Densitometric analysis of 4E-BP1 phosphorylation (upper protein band) or total 4E-BP1 levels in idiopathic or G2019S PD brains compared to control brains. The levels of phosphorylated 4E-BP1 were normalized to total 4E-BP1, whereas total 4E-BP1 levels were normalized to β-actin levels, and expressed as a percent of control subjects (mean±SEM, <i>n</i> = 4–5 brains/group). For basal ganglia, G2019S subject 3 was excluded from the densitometric analysis due to a lack of detectable 4E-BP1 expression. *<i>P</i><0.05 or **<i>P</i><0.01 by one-way ANOVA with Newman-Keuls post-hoc analysis. <i>ns</i>, non-significant.