HCMV inhibits Wnt-3A-induced migration of SGHPL-4 EVTs.

<p>Migration was assayed using BD FluoroBlok transwell inserts. SGHPL-4 cells were infected with Towne-GFP (MOI of 1–2) or mock-infected for 48 hr prior to the assay. Equal numbers of cells were loaded into the inserts and 150 ng/ml of Wnt-3A or PBS were added to both the upper and lower chambers of the transwell system as indicated. Each condition was performed in triplicate. Migrated wells were stained with calcein AM and visualized with a fluorescent microscope. Average fluorescence intensity was determined by capturing three random fields from each well and measuring their fluorescence intensity using ImageJ software. Data are represented as the mean ± SEM (n = 9). **<i>p</i><0.001.