Transwell migration and invasion assays.

<p>(A, B) Transwell migration assay. Representative microscopic images of cells that migrated through the transwell in the migration assay. (Giemsa stain, magnification ×200). No significant differences were observed between the FL subline and parental cell line for A549 or H441 cells. The quantitation of cells that migrated through the transwell in the migration assay. The data are shown as the box-and-whisker plot of the mean number of migrated cells per visual field (A) or 3 visual field (B) (magnification ×100) of 4 replicate wells. No significant differences were found between the FL subline and parental cell line for A549 cells or for H441 cells. (C, D) Transwell Matrigel invasion assay. Representative microscopic images of cells that invaded through the transwell in the Matrigel invasion assay. (Giemsa stain, magnification ×200). No significant differences were observed between the FL subline and parental cell line for A549 or H441 cells. Quantitation of cells that invaded through the transwell in the Matrigel invasion assay. The data are shown as the box-and-whisker plot of the mean number of cells per visual field (C) or 3 visual field (D) (magnification ×100) of 4 replicate wells. The results obtained were similar between A549 and A549-FL cells. Invasion was slightly weaker in H441-FL cells than in H441 cells. Statistical analysis was performed by the Mann-Whitney U test.