STING restricts replication of DENV in mouse cells.

<p>Sting ^+/+ and Sting ^−/− MEFs were infected with DENV-2 (16681 strain) (A, B and C) and DENV-2 (NGC strain) (D, E and F) at an MOI of 5 and total RNA was extracted at the indicated times post infection. Levels of IFNβ were measured by ELISA(A and D) and DENV RNA levels were measured by qRT-PCR (B and E) using 18S and β-actin for normalization.. Infectious particles released to the supernatants of infected MEFs were measured by plaque assay on BHK cells (C and F) as described in materials and methods. Data are representative of two independent experiments. Error bars represent standard deviations of the mean of two replicate samples. <b>*</b>, p<0.05.