DCFDA/DHE-fluorescence in young and old mice.

<p>Myocardial tissue sections (5 µm-thick) on slides were treated with cell-permeable fluorescent H₂DCFDA (A) and DHE (B). The microscopic images were obtained at a magnification of 60X. The oxidized, fluorescent 2-hydroxyethidium and DCF was imaged using confocal microscopy and quantified using Simple PCI 6 Imaging Software. Increased superoxide and hydrogen peroxides were observed in old heart tissues under basal and EES conditions. Green-DCF, Red-DHE and Blue-DAPI (nucleus stain). Respective intensity calculations were shown in the bar-graphs. *p<0.001- between basal and EES; #p<0.001- between young and old.