Representative images and summarized results of superoxide production measured by (A&B) DHE fluorescence in the <i>en face</i> endothelium of aorta and (C) lucigenin-enhanced chemiluminescence method in the aorta of C57BL/6J mice of all groups.

<p>Red: DHE fluorescence (excitation: 515 nm) in the nucleus. Green: autofluorescence of elastin underneath the endothelium (excitation: 488 nm). Lower panel, merged. Bar: 100μm. The NADPH oxidase inhibitor diphenyleneiodonium (DPI, 10 mM) abolished the generation of superoxide anion. Results are means + SEM of 6–7 separate experiments. *P<0.05 compared with control, # P<0.05 compared with tunicamycin.