<p>Cultures were grown with the indicated amounts of C16∶1▵6 (sapienic acid), C16∶1▵9 (palmitoleic acid), C16∶0 (palmitic acid), C18∶2 (linoleic acid), C18∶1 (oleic acid), C18∶3 (linolenic acid) or C18∶0 (stearic acid) fatty acids. Proteins in the cell-free culture supernatant were precipitated in ice-cold TCA, and after solubilization in SDS-PAGE reducing buffer, protein equivalent to 2.0 OD<sub>600</sub> units of culture supernatant was loaded in each lane (A and C). For Western blot (C), 0.02 OD<sub>600</sub> units of cell free culture supernatant were subjected directly to SDS-PAGE, prior to detection with specific antisera (see Materials and Methods).</p
<p>Wild-type, Δ<i>sty1</i> and Δ<i>atf1</i> cells were grown in YES medium. Cell lysates were prepar...
<p>Lane M: protein standards; lane 1, 2: SDS–PAGE analysis of the induced whole cellular lysate; lan...
<p>(A) Western blot analyses of the parental (WT), knock-out and tetracycline-induced (.i) or non-in...
<p>For (A), protein loading was 2.0 OD<sub>600</sub> units for Coomassie staining, and 0.02 OD<sub>6...
<p>Protein loading was 2.0 OD<sub>600</sub> units for SDS-PAGE, and 0.02 OD<sub>600</sub> units for ...
<p>HeLa cells (A) were mock transfected (Mock) or transfected with human recombinant αTFP plasmid (P...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...
<p>M protein molecular weight marker (the size of each band was indicated on the left); Lane 1 cell ...
<p>(A) A 12-h grown culture of indicated strains were grown with agitation. Protein samples were pre...
<p>A 10–20% gradient SDS-PAGE separated crude <i>S. aureus</i> antigen preparations subsequently tra...
<p>The level of secreted proteins in culture supernatant was measured by enzyme-linked immunosorbent...
<p>β-galactosidase reporter gene assay in cell lysate of USA300<i>aur</i> after growth for 5–8 h in ...
Extracellular vesicle preparation western blot profiles of somatic (10 μg protein), excretory-secret...
<p>Arrows indicate the protein of interest. Other bands are the result of non-specific binding. The ...
<p>Western-blot assay of secreted (<b>left</b>) and cell associated (<b>right</b>) toxin components ...
<p>Wild-type, Δ<i>sty1</i> and Δ<i>atf1</i> cells were grown in YES medium. Cell lysates were prepar...
<p>Lane M: protein standards; lane 1, 2: SDS–PAGE analysis of the induced whole cellular lysate; lan...
<p>(A) Western blot analyses of the parental (WT), knock-out and tetracycline-induced (.i) or non-in...
<p>For (A), protein loading was 2.0 OD<sub>600</sub> units for Coomassie staining, and 0.02 OD<sub>6...
<p>Protein loading was 2.0 OD<sub>600</sub> units for SDS-PAGE, and 0.02 OD<sub>600</sub> units for ...
<p>HeLa cells (A) were mock transfected (Mock) or transfected with human recombinant αTFP plasmid (P...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...
<p>M protein molecular weight marker (the size of each band was indicated on the left); Lane 1 cell ...
<p>(A) A 12-h grown culture of indicated strains were grown with agitation. Protein samples were pre...
<p>A 10–20% gradient SDS-PAGE separated crude <i>S. aureus</i> antigen preparations subsequently tra...
<p>The level of secreted proteins in culture supernatant was measured by enzyme-linked immunosorbent...
<p>β-galactosidase reporter gene assay in cell lysate of USA300<i>aur</i> after growth for 5–8 h in ...
Extracellular vesicle preparation western blot profiles of somatic (10 μg protein), excretory-secret...
<p>Arrows indicate the protein of interest. Other bands are the result of non-specific binding. The ...
<p>Western-blot assay of secreted (<b>left</b>) and cell associated (<b>right</b>) toxin components ...
<p>Wild-type, Δ<i>sty1</i> and Δ<i>atf1</i> cells were grown in YES medium. Cell lysates were prepar...
<p>Lane M: protein standards; lane 1, 2: SDS–PAGE analysis of the induced whole cellular lysate; lan...
<p>(A) Western blot analyses of the parental (WT), knock-out and tetracycline-induced (.i) or non-in...