Extracellular vesicle protein preparation Western blot profiles purified using differential centrifugation and size exclusion chromatography.

Extracellular vesicle preparation western blot profiles of somatic (10 μg protein), excretory-secretory protein (10 μg protein), soluble extracellular vesicle protein (20 μg protein) and insoluble extracellular vesicle protein (20 μg protein). Biological triplicates were analysed on 12.5% SDS-PAGE electrophoresis gels and transferred to nitrocellulose membranes for immune recognition. Samples of extracellular vesicle preparations purified using either the differential centrifugation method or size exclusion chromatography method were probed with anti-glutathione transferase sigma class (Anti-FhGST-S1), Anti-Fasciola cathepsin L1 (Anti-FhCat-L1) or anti-fatty acid binding protein V (Anti-FhFABP-V).