Effects of different inhibitors on primary root elongation.

<p>a–d. Cold-pretreated seeds were cultured in 1/2 MS with 1% Suc for 24 h, and then uniform seedlings of similar size and primary root length were transferred to the same fresh medium containing 0 (a), 20 µM MG132 (b), 40 µM MG132 (c) or 80 µM MG132 (d), respectively, for additional 48 h. Bars = 1000 µm. e–h. Cold-pretreated seeds were cultured in 1/2 MS with 1% Suc for 24 h, and then uniform seedlings of similar size and primary root length were transferred to the same fresh medium containing various concentrations of MG 132 (e), MG115 (f), E-64 (g) and DMSO (h), for additional 48 days. The values are the means ± SD of about 15 seedlings of each treatment. The experiment was repeated thrice with consistent results.