Immunoblot analysis of sucrose-gradient fraction 2 and 3 of WT and the RpaA inactivation mutant.

<p><b>A</b>. F2, which contains PS II and monomeric PS I complexes, was collected and separated by a 12%–20% SDS-PAGE with 6 M urea. PsaA, PsaD and D1 were identified using corresponding polyclonal antibodies. <b>B</b>, The PsaA, PsaD, PsbA and CP47protein of the F3 (containing PS I trimers) was analyzed by Western blot using anti-PsaA, PsaD, PsbA and CP47 antibodies. <b>C</b>, The PsaL protein in thylakoid membranes was estimated using anti PsaL antibody.