TG2 Inhibitors Differentially Affect TG2 Conformation.

<p>(A) Quantitative FLIM-FRET data shown as percent efficiency of FRET for the mCerulean-TG2-eYFP FRET sensor after a 16 hour treatment with either the irreversible TG2 inhibitor, NC9, or the reversible TG2 inhibitor, CP4d. (B) Percent efficiency of FRET graph generated following treatment of TG2 sensor with 5 increasing concentrations of CP4d. (C) Percent efficiency of FRET graph for TG2 sensor following treatment with 10 µM NC9 for 5 different time points from 0 to 24 hours. Black line represents median values, boxes encompass 25% and 75% confidence intervals and whiskers indicate the 5% to 95% confidence intervals. *p<0.001, **p<0.05 and N.S = not significant. N>10 for 4 replicates.