Point Mutations and Cell Stresses Affect TG2 Conformation in Live Cells.

<p>(A) Quantitative FLIM-FRET data shown as percent efficiency of FRET for the mCerulean-TG2-eYFP FRET sensor, GDP/GTP insensitive R580A mutant and catalytically inactive W241A mutant. Black line represents median values, boxes encompass 25% and 75% confidence intervals and whiskers indicate the 5% to 95% confidence intervals. *p<0.001. N = 15 for 4 replicates. (B–D) Representative FLIM images of data in (A). (E) Quantitative FLIM-FRET data shown as percent efficiency of FRET for the mCerulean-TG2-eYFP FRET sensor, or the sensor following either a 30 minute heat shock (42.5°C) treatment or a treatment with Ca²⁺ ionophore A23187 for 10 minutes. (F, G) Representative FLIM images of data in (E). *p<0.001. N>10 for 4 replicates. Scale bar represents 10 µm.