α-Synuclein is not an open-channel blocker to hα4β2-nAChR.

<p><b>A</b>. Representative hα4β2-nAChR mediated whole-cell currents induced by repetitive applications nicotine (4 sec exposure at an interval of 2 min). The first response was recorded as controls. In <b>Aa</b>, nicotine exposures were repeated at 2-min intervals in the presence of 10 nM α-synuclein for 10 min, and subsequent response to nicotine was recorded after 6 min of washout of α-synuclein. In <b>Ab</b>, nicotine was applied at the end of the α-synuclein treatment (10 min), and a subsequent response to nicotine was recorded after 6 min of washout of α-synuclein. <b>B</b>. Bar graph summarizes replicated recordings of effects of 10 nM α-synuclein on nicotinic responses with and without repeated nicotine exposure. Data were collected from 8 cells in each group tested.