The activation state of AMPK.

<p>(<b>A</b>) Representative blots of AMPK-P (T172), AMPKα1 and AMPKα2 isoforms. 1 hour CLFS samples on the left and 24 hours CLFS on the right. Samples are C: The correspondent non-stimulated control, and 0 h, 1 h, 6 h, 12 h and 24 h are the time of rest after the stimulation. (<b>B</b>) Quantification of the AMPK-P (T172) Western-blot blot normalized by AMPKα1 in fold change (from 0 to 160 fold) compared to control and in a different scale (<b>C</b>) with a break from 7 to 35 to better display the data in the control samples and samples rested from 6 to 24 hours. Quantification of AMPKα1 (<b>D</b>) and AMPKα2 (<b>E</b>) in fold change compared to control from the Western-blot normalized by total protein content. 1 hour (▪) and 24 hours (▪) stimulated samples n = 5. Control group (□) was composed with the pool of all contralateral non-stimulated muscle samples results (n = 50). Data are means ± SE. Bars with the same letter are not significantly different from each other.