Xn and Xc activate AMPK via the LKB1 signaling pathway.

<p>(a) Indicated agents were treated for 5 min and 5 µM concentration with or without 30 min pre-incubation of 2.5 µM concentration of STO609 in L6 myotubes. (b) L6 myotubes was transfected by mock or two different LKB1 siRNA for 48 h. After infection, indicated agents were treated for 5 min and 5 µM concentration. The graphs show quantification of western blot based on densitometric analysis. (c) Indicated agents were treated for 5 min and 5 µM concentration to L6 myotubes. Celluar AMP/ATP ratio was measured by high-performance liquid chromatography as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108771#s2" target="_blank">Materials and Methods</a>. Western blot data represent one of three independent experiments. Values are mean ± SE. of three independent experiments. N.S means not significant. *, <i>P</i><0.05 and **, <i>P</i><0.01 versus non-treated controls.