DPSC osteoblastic and myocytic differentiation characterization.

<p>(A) Evidence of the osteo-differentiated DPSC morphology change, bar scales 150 μm. (B) Semiquantitative rt-PCR was used to determine the gene expression profiles ratio of osteoblastic specific markers in 1 month osteoblastic-differentiated over undifferentiated cells, relative to housekeeping genes. Values were expressed as mean ± SD and were normalized using β-actin. X-axis shows relative expression fold Y-axis tested markers, (adjusted <i>P</i><0.05). (C) FITC labeled antibody was used to evaluate, by IF, osteocalcin expression in 1 month osteoblastic-differentiated DPSC, bar scales 75 μm. (D) FITC labeled antibody was used to evaluate, by IF, ostepontin expression in 1 month osteoblastic-differentiated DPSC, bar scales 75 μm. Nuclei were counter stained with DAPI. (E) X-ray powder diffraction patterns from the DPSC aggregates after 3 months of osteoblastic induction. The main diffraction peaks of carbonate hydroxyapatite (#) and sodium chloride (°) and the wave numbers of the absorption peaks are indicated. (F) The FTIR spectrum shows absorption peaks at the characteristic peaks of phosphate group (ν³ = 1037 cm-1; ν⁴ = 603 cm⁻¹ and 564 cm⁻¹) and carbonate group (ν³ = 1421 cm⁻¹). (G) Evidence of the myocyte-differentiated DPSC morphology change, bar scales 150 μm. (H) Semiquantitative rt-PCR was used to determine the gene expression profiles ratio of myocytic specific markers in 1 month myocyte-differentiated over undifferentiated DPSC cells. Values were expressed as mean ± SD and were normalized using β-actin. X-axis shows relative expression fold Y-axis tested markers, (adjusted <i>P</i><0.05). (I) TRITC labeled antibody was used to evaluate, by IF, sarcomeric actin expression in 1 month myocyte-differentiated DPSC, bar scales 25 μm. (J) FITC labeled antibody was used to evaluate, by IF, connexin 43 expression in 1 month myocyte-differentiated DPSC, bar scales 25 μm. (K) FITC labeled antibody was used to evaluate, by IF, ATPase pump serca 2 expression in 1 month myocyte-differentiated DPSC, bar scales 75 μm. L: FITC labeled antibody was used to evaluate, by IF, smooth muscle actin expression in 1 month myocyte-differentiated DPSC, bar scales 25 μm. Nuclei were counter stained with DAPI.