TLR2/1L-induced expression of human host defence peptide LL-37 in macrophages.

<p>MDMs were stimulated with TLR2/1L (10 µg/ml) in the presence of autologous serum. (<b>A</b>) Gene expression of LL-37 was monitored by qRT-PCR after 6 hr. Fold changes (y-axis) was normalized to 18S RNA and is represented relative to gene expression in un-stimulated cells normalized to 1 using the comparative Ct method. Results represent an average of five independent experiments (MDMs isolated from five Dené and five Caucasian participants) ± standard error (*p<0.05 and NS = non-significant). (<b>B</b>) LL-37 peptide expression was monitored by probing immunoblots with anti-LL-37 antibody after 21 hr. The immunoblot shown is a representative blot of 4 independent experiments from MDMs isolated from independent Dené and Caucasian participants each.