TLR2/6 ligand lead to an increase of hCAP18/LL-37 gene expression by activation of Cyp27B1 and VDR.

<p>(A)Human macrophages were pretreated with the VDR antagonist ZK159222 (10⁻⁷ M) or the Cyp27B1 antagonist itraconazole (10⁻⁷ M) for 2 h and then stimulated with TLR2/6 ligand Pam2CSK4 (100 ng/ml) in the presence or absence of 25D3 (10⁻⁶ M) (DMEM without serum) for 24 h. Expression of hCAP18/LL-37 mRNA was determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056616#pone-0056616-g003" target="_blank">Figure 3</a>. (B) Regulation of hCAP18/LL-37 gene on stimulation with 25D3 in DMEM (without serum) or SKOV3-CM (without serum). Mean fold change ± SEM, n = 3, *p<0.05.