Effects of Ang-(1-7) on AngII-stimulated intracellular ROS production.

<p>A, Confocal microscopic images of cells subjected to DCF-DA treatment. Cells were exposed to Ang II (10⁻⁶ M) for 24 h with or without Ang-(1-7) (10⁻⁶ M), A779 (10⁻⁶ M), and apocynin (100 µM). B Measurement of DCF-DA fluorescence intensity. C, Flow cytometric analysis of cells treated with DCF-DA after treatment with Ang-(1-7) plus Ang II, reflecting a reduction in ROS generation. Values are the mean ± S.E.M. (n = 4). *p<0.05 versus control; ^#p<0.05 versus Ang II-stimulated cells.