PCR and Southern blot analysis of transgenic cotton.

<p>A. PCR-based verification of stable insertion of the <i>npt II</i> gene into the genomic DNA of cotton using an <i>npt II -</i>specific probe; and B, of the <i>bel</i> gene with a <i>bel-</i>specific probe, C. Southern blot of T1 plants, Lane 1 shows positive control plasmid DNA, D. <i>Bel</i> expression analysis of RT-PCR of six plants of individual T2 and T3 plants, using <i>actin</i> as internal control. (Lane 1 showing Coker 312 as a negative control, Lane 2–7 showing T2 plants, Lane 8–13 showing T3 plants generated from T2 plants from plants in Lanes 2–7; <i>Actin</i> gene in cotton as reference gene).