Effect of miR-195 on cell invasion in HTR8/SVneo cells.

<p>A and D. The expression of miR-195 in HTR8/SVneo cells transfected with miR-195 mimics (miR-195), scramble siRNA (NC), miR-195 antigomir (Anti-miR-195) or scramble antigomir (Inhibitor NC) was measured by real time PCR. The level of miR-195 was adjusted by that of U6, and the relative value was presented as Mean±SEM according to three independent experiments. *, compared with corresponding NC, <i>p</i><0.05. B and E. Transwell insert assay to examine cell invasiveness transfected with NC, miR-195, siRNA for ActRIIA (si-ActRIIA), Inhibitor NC or Anti-miR-195. The value of invasion index in corresponding NC was set as 100, and the results were presented as Mean±SEM according to three independent experiments. *, compared with corresponding NC, <i>p</i><0.05. C and F. Gelatin zymography to measure MMP-2 and MMP-9 productions in cells transfected with miR-195, NC, Inhibitor NC or Anti-miR-195. Upper panels, typical results of gelatin zymography. Conditioned media of HT1080 cells was included as positive control for zymography. The pro- and active forms of MMP-2 and MMP-9 were separately indicated. Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of pro- and active forms of MMP-2 and MMP-9 were presented as Mean±SEM. *, compared with data in corresponding NC, <i>p</i><0.05.