Short time effect (0–4 h) of the AMPK inhibition in the percentage of rapid spermatozoa.

<p>Spermatozoa were incubated in TBM (A, circles) or TCM medium (B, squares) at 38.5°C in the absence (white) or presence (black) of the AMPK inhibitor, compound C, (CC 30 µM) during 4 h. Samples at 17°C were considered as time 0. The percentage of those motile spermatozoa with VAP>80 µm/s, defined as rapid spermatozoa, was measured. This experiment was performed at least 6 times and the results express the percentage of rapid spermatozoa from the total spermatozoa motile analyzed (4.000–5.000). Statistical differences were considered when p<0.05 and showed with an asterisk.