Effect of AMPK inhibitor CC and activators AICAR and MET on ATP concentration in frozen-thawed sperm.

<p>Sperm were treated in the presence of an AMPK activator (2mM AICAR (in light gray) or 1mM MET (in diagonals)) or an AMPK inhibitor (5μM CC (in dark gray)) or Control (Ctrl, in white); Fresh semen (checkered pattern). Values represent means ± SEM from 6 different experiments (4 samples/experiment). Different superscripts indicate significant differences between Ctrl and treatments (AICAR, MET or CC) in frozen-thawed semen (^a,b,c, P<0.05). Asterisks indicate statistically significant differences between fresh and frozen-thawed semen (*, P<0.001).