Acute GM exposure decreased NADH Fl in basal turn OHCs bathed in T1 imaging buffer.

<p>A) I/OHCs in apical, low-frequency regions of the cochlea do not show significant alterations in NADH Fl during acute GM exposure (n_GM = 5, n_Cont = 4). A transient difference was observed in OHCs at 60 min (t(7) = 1.96, p<0.05). B) Although basal turn, high-frequency IHCs were unaltered by GM, basal turn OHCs displayed a significant decrease in NADH Fl within 10 min of GM exposure (t(7) = 2.0, p<0.05, n_GM = 5, n_Cont = 4). C) Representative image of NADH Fl intensity in basal turn I/OHCs before and after (D) GM exposure. The location of the IHC row and OHC rows are indicated by circling an individual HC from each location. T1 imaging buffer contained 5 mM glucose. Scale bar = 10 µm. * = p<0.05, ** = p<0.01.