T2 buffer increased NADH reduction/production but failed to prevent the GM NADH effect.

<p>A) Apical turn I/OHCs display transient, significant increases in NADH Fl when bathed in T2 buffer (n_T1 = 5, n_T2 = 4). B) Basal turn OHCs display a significant and prolonged increase in NADH Fl in T2 buffer while basal turn IHCs do not (n_T1 = 5, n_T2 = 4). C) T2-bathed, apical turn I/OHCs display moderate decreases in NADH Fl during acute GM exposure (n_GM = 4, n_Cont = 5). D) T2-bathed basal turn OHCs, not basal turn IHCs, display significant and prolonged decreases in NADH Fl during GM exposure (n_GM = 5, n_Cont = 5). T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate. * = p<0.05, ** = p<0.01.