Microglia are less reactive in Gfap^+/R236H mice without Nrf2.

<p>(<b>A</b>) Compared to wild-type mice, Gfap mutant mice show elevated Iba1 immunoreactivity in hippocampus either with or without Nrf2. (<b>B</b>) Western analysis of hippocampal protein shows reduced Iba1 expression in Gfap^+/R236H/Nrf2^−/− compared with Gfap^+/R236H/Nrf2^+/+ mice (8 wks), although levels remain elevated above wild-type (**p<0.01, unpaired t-test comparing Gfap^+/R236H/Nrf2^+/+ with Gfap^+/R236H/Nrf2^−/− mice, n = 4). Lanes 5 and 6 for Nrf2^−/− samples are from a different region of the same gel as lanes 1–4 (Nrf2^+/+ samples).