Mutagenesis analyses of the predicted epitope residues.

<p><b>A</b>. The reactivity of AVFluIgG01 with wild-type (WT) HA or HA containing site-directed mutations analyzed by flow cytometry. <b>B</b>. The reactivity of AVFluIgG01 with the WT (red curve) or HA carrying single mutations (green curve) analyzed by immuofluorescence assay (IFA). In both assays, the WT HA or mutants were transiently expressed on 293T cells by transfection and then immunostanined by AVFluIgG01 or a control mAb at a final concentration of 10 µg/ml.