Relative extent of granular area within pancreatic acinar cells of wild-type and knockout mice.

<p><i>A, C</i>. Cross-sectional fluorescence images of wild-type (WT; A) and knockout (KO; C) mouse acinar cells loaded with fura-2 FF-AM. N indicates nucleus. <i>B, D</i>. Fura-2 FF fluorescence intensity of WT (B) and KO (D) acinar cells, as measured along the solid white lines on the images A and C, respectively. <i>E</i>. Plot of cell-size against “granular area proportion” in WT (black dots) and KO (red dots) acinar cells. “Granular area proportion” was calculated by expressing the extent of the low-intensity area as a percentage of the cell area (namely, the sum of low-intensity and high-intensity areas) on the basis of the above findings derived from cross-sectional images. Dots labeled A and C correspond to the cells in images A and C, respectively.