Detailed gene amplification analysis on human chromosome 16 and 12.

<p>Representative sections of log₂ ratio profiles for undifferentiated (0 d) NHNP cells and cells that were differentiated for 2 and 5 days. Base count is given on the x-axis and log₂ ratio on the y-axis for chromosome 16q24.1 (A) and 12q14.1 (C). Chromosomal localization of BAC probes used for FISH were indicated at the bottom of figures C and D. A <i>GINS2</i> specific BAC probe that was labeled in pink and a <i>CDH13</i> specific BAC probe that was labeled in green were hybridized simultaneously against fixed NHNP cells that were differentiated for 2 days. <i>GINS2</i> amplification is indicated as pink speckled fluorescence signals whereas the neighboring <i>CDH13</i> gene shows only single copy fluorescence signals (Bi). Neighboring cells with and without <i>GINS2</i> amplification are shown in Figure Bii. A <i>CDK4</i> specific BAC probe that was labeled in pink and a <i>XRCC6BP1/KUB3</i> specific BAC that was labeled in green were hybridized simultaneously against NHNP cells that were differentiated for 2 days. <i>CDK4</i> and <i>KUB3</i> amplifications were detectable as cluster of pink and green speckled fluorescence signals. <i>CDK4</i> specific signals spread over a more extended area than the <i>KUB3</i> specific signals (D). Nuclei were counterstained with DAPI (B). Size calibration bar = 5 µm.