Attenuation of the severity of somatic signs of naloxone-precipitated morphine withdrawal up to 30 min after the naloxone injection by SB-334867 pretreatment.

<p>The following variables were counting: (A) wet-dog shakes; (B) sniffing; (C) writhing; (D) body tremor; (E) ptosis; (F) diarrhoea; (G) piloerection; (H) mastication; (I) teeth chattering; (J) paw tremor; (K) body weight loss. Somatic signs of withdrawal were observed during 30 min immediately after naloxone injection (1 mg/kg s.c.) A global withdrawal score (L) was calculated for each animal as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036871#s4" target="_blank">Methods</a>. Data are expressed as mean ± SEM. *p<0.05; ***p<0.001, versus placebo + vehicle (veh) + naloxone (nx); ⁺⁺p<0.01; ⁺⁺⁺p<0.001 versus similar groups receiving vehicle instead of SB-334867.