The chemical structure of inotilone (A) and the effects of inotilone on lipopolysaccharide (LPS)-induced cell viability (B), NO production (C), inhibition of iNOS and COX-2 protein expression (D), and MAPK (JNK, p38, and ERK) protein expression (E) were evaluated in RAW264.7 cells.

<p>Cells were incubated for 24 h or 5, 10, 15, 30, and 60 mins with 100 ng/mL of LPS in the absence or presence of inotilone (0, 1.56, 3.12, 6.25, 12.5, and 25 µM). Inotilone was added 1 h before the incubation with LPS. Cell viability was performed by using MTT assay. Nitrite concentration in the medium was determined by using Griess reagent. Lysed cells were then prepared and subjected to Western blotting by using an antibody specific for iNOS, COX-2, and MAPK. β-actin was used as an internal control. The data were presented as mean ± S.D. for three different experiments performed in triplicate. **<i>p</i><0.01 and ***<i>p</i><0.001 were compared with LPS-alone group.